03172nam a22004934a 4500999001700000001000600017003000900023005001700032008004100049010001700090020003500107020001800142040006200160050002100222060001500243060002400258070002100282082001500303100002700318245008300345250001600428260007600444300004700520500017700567504006900744505115100813526000801964650003201972650002102004650002502025650003202050650003202082650002602114650003102140650002902171650002102200650002102221700002702242700002302269852004002292942001202332952017302344952016102517  c39303d3930333063BD-DhAAL20220113112619.0180506r20142006us a     b    001 0 eng    a  2005018202  a1405135441 (pbk. : alk. paper)  a9788126548392  aDNLM/DLCcDLCdNLMdBAKERdBWKUKdUKMdAGLdDLCdBD-DhAAL00aQH442b.O42 200600a2006 H-69010aQH 442bP952pa 20060 aQH442b.O42 200600a660.652221 aPrimrose, S. B.92574810aPrinciples of gene manipulation and genomics /cS.B. Primrose and R.M. Twyman.  a7th edition  aNew Delhi ;aHoboken, NJ :bBlackwell Publishing,cc2006 [reprint 2014]  axxii, 644 pages :billustrations ;c28 cm.  aRev. ed. of: Principles of gene manipulation.7th ed. 2006 and Principles of genome analysis and genomics / Sandy B. Primrose, Richard M. Twyman.. 6th ed. 2001.3rd ed. 2003.  aIncludes bibliographical references (pages [547]-626) and index.0 aGene manipulation in the post-genomics era -- Basic techniques -- Cutting and joining DNA molecules -- Basic biology of plasmid and phage vectors -- Cosmids, phasmids, and other advanced vectors -- Gene cloning strategies -- Sequencing genes and short stretches of DNA -- Changing genes : site-directed mutagenesis and protein engineering -- Bioinformatics -- Cloning in bacteria other than Escherichia coli -- Cloning in Saccharomyces cerevisiaea and other fungi -- Gene transfer to animal cells -- Genetic manipulation of animals -- Gene transfer to plants -- Advanced transgenic technology -- The organization and structure of genomes -- Mapping and sequencing genomes -- Comparative genomics -- Large-scale mutagenesis and interference -- Analysis of the transcriptome -- Proteomics I : expression analysis and characterization of proteins -- Proteomics II : analysis of protein structures -- Proteomics III : protein interactions -- Metabolomics and global biochemical networks -- Applications of genomics : understanding the basis of polygenic disorders and identifying quantitative trait loci -- Applications of recombinant DNA technology.  aAAL 0aGenetic engineering.925749 0aGenomics.925750 0aGene mapping.925751 0aNucleotide sequence.92575212aGenetic Engineering.92575322aBase Sequence.92575422aChromosome Mapping.92575522aDNA, Recombinant.92575622aGenomics.92575022aPharmacy.9449701 aPrimrose, S. B.9257581 aR.M. Twyman925759  aAyesha Abed LibrarycGeneral Stacks  2ddccBK  00102ddc4070aBRACULbBRACULcGENd2018-04-25eTrim Educationg3575.00l5m84o660.65 PRIp3010033064q2026-04-27r2025-05-05s2025-05-05t2v3575.00w2018-04-25yBK  00102ddc4070aBRACULbBRACULcGENd2018-04-25eTrim Educationg3575.00l6m37o660.65 PRIp3010033063r2025-05-24s2025-05-07t1v3575.00w2018-04-25yBK