000			02933nam a22003978i 4500
001			44451
003			BD-DhAAL
005			20250721155119.0
008			250616t2021 njuab b 001 0 eng
010			_a 2020029236
020			_a9781119640783
020			_a9781119640752
020			_a9781119640677
040			_aDNLM/DLC _beng _erda _cDLC _dBD-DhAAL
042			_apcc
050	0	0	_aQH442.2
082	0	0	_a572.8633 _223
100	1		_aBrown, T. A. _q(Terence A.), _eauthor.
245	1	0	_aGene cloning and DNA analysis : _ban introduction / _cTerry A. Brown.
250			_aEighth edition.
260			_aHoboken, NJ : _bWiley-Blackwell, _c2021.
300			_a xv, 416 pages : _billustrations (chiefly color), color maps ; _c25 cm.
504			_aIncludes bibliographical references and index.
505	0		_aWhy gene cloning and DNA analysis are important -- Vectors for gene cloning : plasmids and bacteriophages -- Purification of DNA from living cells -- Manipulation of purified DNA -- Introduction of DNA into living cells -- Cloning vectors for Escherichia coli -- Cloning vectors for eukaryotes -- How to obtain a clone of a specific gene -- The polymerase chain reaction -- Sequencing genes and genomes -- Studying gene expression and function -- Studying genomes -- Studying transcriptomes and proteomes -- Production of protein from cloned genes -- Gene cloning and DNA analysis in medicine -- Gene cloning and DNA analysis in agriculture -- Gene cloning and DNA analysis in forensic science and archaeology.
520			_a"The main development in DNA technology since publication of the Seventh Edition of Gene Cloning has been the increased use of gene editing as a tool in both research and biotechnology. The basic methodology for CRISPR editing is now described in Chapter 12 and the applications of the method are explored, in the context of plant genetic engineering, in Chapter 16. Elsewhere, the continuing evolution of next-generation DNA sequencing is reflected by a reorganization of this part of Chapter 10, and to deal with the further proliferation of methods for studying transcriptomes and proteomes (albeit not strictly DNA Analysis) I have created a new chapter devoted to these methods. Other additions include new sections on melt curve analysis of real-time PCR products and genetic typing of human disease mutations"--
526			_aMNS
650	1	2	_aCloning, Molecular.
650	2	2	_aSequence Analysis, DNA.
650	2	2	_aDNA, Recombinant _xAnalysis.
650	2	2	_aDNA _xAnalysis.
650	2	2	_aNucleotide sequence.
650	2	2	_aMolecular cloning.
852			_aAyesha Abed Library _cGeneral Stacks
942			_2ddc _cBK
999			_c47306 _d47306