Understanding the effect of exposure of human pathogens to polluted surface water: Salmonella as a model organism
This thesis report is submitted in partial fulfilment of the requirement for the degree of B.Sc in Microbiology, 2018.
| Prif Awdur: | |
|---|---|
| Awduron Eraill: | |
| Fformat: | Traethawd Ymchwil |
| Iaith: | English |
| Cyhoeddwyd: |
BRAC Univeristy
2018
|
| Pynciau: | |
| Mynediad Ar-lein: | http://hdl.handle.net/10361/10307 |
| id |
10361-10307 |
|---|---|
| record_format |
dspace |
| spelling |
10361-103072019-09-30T04:56:06Z Understanding the effect of exposure of human pathogens to polluted surface water: Salmonella as a model organism Munir, Maliha Nuzhat Siddiqee, Mahbubul Hasan Department of Mathematics and Natural Sciences, BRAC University Salmonella DNA extraction Plasmid profiling This thesis report is submitted in partial fulfilment of the requirement for the degree of B.Sc in Microbiology, 2018. Catalogued from PDF version of thesis report. Includes bibliographical references (pages 34-37). Unplanned urbanization often results in the surface waters getting contaminated with industrial and faecal pollutants. The wide spectrum of industrial pollutants put pressure on the faecal organisms during their survival in these polluted waterbodies. Although such pressure is commonly known to result in shorter survival of these organisms which can be pathogenic to humans, its effect on the virulence indicators is not fully understood. This study attempts to detect the presence and responses of Salmonella in waterbodies contaminated with industrial pollutants. A lake (Hatirjheel) receiving low level of industrial pollutants and a river (Buriganga) receivng high level of industrial pollutants within Dhaka city were selected for sampling. A total of 40 water samples were aseptically collected from Hatirjheel Lake and 20 samples from Buriganga River within a duration of three months. Then they were processed for isolation of cultureable Salmonella, before checking for Salmonella specific virulence gene invA. After that, randomly selected 5 strains from each were checked for their susceptibility to an array of eight antibiotics. Further, randomly selected three strains from each lake were tested for survival potential under starvation stress. Across the study period, 40% of the sample collected from the lake and 70% of the sample collected from the river yielded culturable Salmonella all of which contained invA gene. The antibiogram revealed that isolates from Buriganga showed complete resistance to ampicillin, co-trimoxazole and cefepime while the Hatirjheel isolates showed complete resistance to only ampicillin and cefepime. The survival patterns between the lake and river showed slight difference but both showed a gradual decrease in culturable count over the time. The study reveals that both Buriganga River and Hatirjheel Lake can be endemically contaminated with potentially pathogenic Salmonella and that there is an indication that the pollution level might have some influence on antibiotic resistance of Salmonella. To the best of our knowledge, this is the first study on focusing on Salmonella in the surface waters of Dhaka city. Maliha Nuzhat Munir B. Science in Microbiology 2018-07-03T10:02:02Z 2018-07-03T10:02:02Z 2018 2018 Thesis ID 14126006 http://hdl.handle.net/10361/10307 en BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 37 pages application/pdf BRAC Univeristy |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Salmonella DNA extraction Plasmid profiling |
| spellingShingle |
Salmonella DNA extraction Plasmid profiling Munir, Maliha Nuzhat Understanding the effect of exposure of human pathogens to polluted surface water: Salmonella as a model organism |
| description |
This thesis report is submitted in partial fulfilment of the requirement for the degree of B.Sc in Microbiology, 2018. |
| author2 |
Siddiqee, Mahbubul Hasan |
| author_facet |
Siddiqee, Mahbubul Hasan Munir, Maliha Nuzhat |
| format |
Thesis |
| author |
Munir, Maliha Nuzhat |
| author_sort |
Munir, Maliha Nuzhat |
| title |
Understanding the effect of exposure of human pathogens to polluted surface water: Salmonella as a model organism |
| title_short |
Understanding the effect of exposure of human pathogens to polluted surface water: Salmonella as a model organism |
| title_full |
Understanding the effect of exposure of human pathogens to polluted surface water: Salmonella as a model organism |
| title_fullStr |
Understanding the effect of exposure of human pathogens to polluted surface water: Salmonella as a model organism |
| title_full_unstemmed |
Understanding the effect of exposure of human pathogens to polluted surface water: Salmonella as a model organism |
| title_sort |
understanding the effect of exposure of human pathogens to polluted surface water: salmonella as a model organism |
| publisher |
BRAC Univeristy |
| publishDate |
2018 |
| url |
http://hdl.handle.net/10361/10307 |
| work_keys_str_mv |
AT munirmalihanuzhat understandingtheeffectofexposureofhumanpathogenstopollutedsurfacewatersalmonellaasamodelorganism |
| _version_ |
1814309415179780096 |