Isolation, molecular characterization and antibiotic resistant of pathogenic bacteria obtained from raw meat in Dhaka city
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018.
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Brac University
2019
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| Διαθέσιμο Online: | http://hdl.handle.net/10361/11862 |
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10361-118622019-09-30T05:19:02Z Isolation, molecular characterization and antibiotic resistant of pathogenic bacteria obtained from raw meat in Dhaka city Tuly, Nurzahan Yasmin Khan, Kashmery Department of Mathematics and Natural Sciences, Brac University Raw meat bacteria Dhaka city Antimicrobial resistance Pathogenic bacteria. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018. Cataloged from PDF version of thesis. Includes bibliographical references (page 49-51). A large number of people intake proteins from meat. But the problem is a number of bacteria are being identified during the several years from raw beef which cause different physical problems and sometimes turns into life-threatening. Improper handling and poor hygiene while handling and preparing meat causes different kinds of health problems in Bangladesh. Transmission of E. coli through the contact with animals and individual to individual via the fecal-oral route has become common. In this study, 24 samples of raw beef were collected from different areas of Dhaka city. After performing biochemical tests Eschrerichia coli, Staphylococcus, Bacillus, and Enterobacteriaceae were found in the selective media also. In this research biochemical tests such as streaking on EMB, Macconkey, XLD, and Nutrient agar medium were done. After that, antibiotic sensitivity testing was done by using 4 antibiotics; they are Kanamycin, Gentamycin, Erythromycin, and Streptomycin. Resistance was found against Erythromycin from 7 samples. Genomic DNA was isolated from samples. Then PCR was done with 16SrRNA and STX 1 separately to identify the presence of STX 1 and STX 2. Gel electrophoresis results showed bands which correspond to the presence of 16SrRNA and STX 1,that signifies the presence of pathogenic bacteria in meat. DNA sequencing of 2 samples showed that they are Enterobacter and Lactobacillus brevis. Nurzahan Yasmin Tuly B. Biotechnology 2019-05-08T06:00:48Z 2019-05-08T06:00:48Z 2018 2018-07 Thesis ID 13336001 http://hdl.handle.net/10361/11862 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 51 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Raw meat bacteria Dhaka city Antimicrobial resistance Pathogenic bacteria. |
| spellingShingle |
Raw meat bacteria Dhaka city Antimicrobial resistance Pathogenic bacteria. Tuly, Nurzahan Yasmin Isolation, molecular characterization and antibiotic resistant of pathogenic bacteria obtained from raw meat in Dhaka city |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018. |
| author2 |
Khan, Kashmery |
| author_facet |
Khan, Kashmery Tuly, Nurzahan Yasmin |
| format |
Thesis |
| author |
Tuly, Nurzahan Yasmin |
| author_sort |
Tuly, Nurzahan Yasmin |
| title |
Isolation, molecular characterization and antibiotic resistant of pathogenic bacteria obtained from raw meat in Dhaka city |
| title_short |
Isolation, molecular characterization and antibiotic resistant of pathogenic bacteria obtained from raw meat in Dhaka city |
| title_full |
Isolation, molecular characterization and antibiotic resistant of pathogenic bacteria obtained from raw meat in Dhaka city |
| title_fullStr |
Isolation, molecular characterization and antibiotic resistant of pathogenic bacteria obtained from raw meat in Dhaka city |
| title_full_unstemmed |
Isolation, molecular characterization and antibiotic resistant of pathogenic bacteria obtained from raw meat in Dhaka city |
| title_sort |
isolation, molecular characterization and antibiotic resistant of pathogenic bacteria obtained from raw meat in dhaka city |
| publisher |
Brac University |
| publishDate |
2019 |
| url |
http://hdl.handle.net/10361/11862 |
| work_keys_str_mv |
AT tulynurzahanyasmin isolationmolecularcharacterizationandantibioticresistantofpathogenicbacteriaobtainedfromrawmeatindhakacity |
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