Determination of bacterial and viral pathogen spectra and identification of multi drugs resistance gene CTX-M1 in Klebsiella pneumoniae isolated from acute respiratory infections children aged under-5 from two hospital settings of Dhaka city
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019.
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Brac University
2019
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10361-120832019-09-30T05:33:11Z Determination of bacterial and viral pathogen spectra and identification of multi drugs resistance gene CTX-M1 in Klebsiella pneumoniae isolated from acute respiratory infections children aged under-5 from two hospital settings of Dhaka city Hossain, Mohammad Amir Choudhury, Dr. Naiyyum Mannor, Dr. Kaiissar Qadri, Dr. Firdausi Department of Mathematics and Natural Sciences, Brac University This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019. Cataloged from PDF version of thesis. Includes bibliographical references (page 69-75). This study aimed to determine bacterial and viral pathogen spectra and identify multidrug resistance genes in bacterial isolated from under-5 children with acute respiratory infections from two hospital settings of Dhaka city. Nasal swabs were collected from 150 under-five children hospitalized with clinical signs of ARIs. Screening of viral pathogens targeted ten respiratory viruses using RT-qPCR. Bacterial pathogens were identified by bacteriological culture methods and antimicrobial susceptibility of the isolates was determined following Clinical and Laboratory Standard Institute (CLSI) guidelines. About 78% (n=117) of specimens were positive for pathogens. Of 117 infected cases, 4.27% (n=5) had only single bacterial pathogens, whereas 44% (n=61) cases had only single viral pathogens. The remaining 58% (n=88) cases had coinfections. In viral suspected cases, human rhinovirus was detected as the predominant virus (32%), followed by RSV (17.33%), HMPV (16%), HBoV (13%), HPIV-3 (12%), and adenovirus (9%). Streptococcus pneumoniae was the most frequently isolated bacterial pathogen (8%), where Klebsiella pneumaniae, Streptococcus spp., Enterobacter agglomerans, and Haemophilus influenzae were 6%, 3.3%, 2%, and 2%, respectively. Of 15 multidrug-resistant bacteria, a Klebsiella pneumoniae (KPn) isolate exhibited resistance against more than 10 different antibiotics and extended spectrum beta-lactamase (ESBL) CTX- M1 gene was found in multidrug-resistant KPn using conventional PCR. Sanger sequencing had been used for confirmation of amplified CTX- M1. Both ARI incidence and pathogen detection rates were higher during post-monsoon and winter, peaking in September. Pathogen detection rates and coinfection incidence in less than 1-year group were significantly higher (P=0.04123) than in 1-5 years age group. Human Boca Virus (HBoV) had a significant involvement between single and confections with P value of 0.0152. Mohammad Amir Hossain B. Biotechnology 2019-05-22T05:30:37Z 2019-05-22T05:30:37Z 2019 2019-01 Thesis ID 14276001 http://hdl.handle.net/10361/12083 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 80 pages application/pdf Brac University |
| institution |
Brac University |
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Institutional Repository |
| language |
English |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2019. |
| author2 |
Choudhury, Dr. Naiyyum |
| author_facet |
Choudhury, Dr. Naiyyum Hossain, Mohammad Amir |
| format |
Thesis |
| author |
Hossain, Mohammad Amir |
| spellingShingle |
Hossain, Mohammad Amir Determination of bacterial and viral pathogen spectra and identification of multi drugs resistance gene CTX-M1 in Klebsiella pneumoniae isolated from acute respiratory infections children aged under-5 from two hospital settings of Dhaka city |
| author_sort |
Hossain, Mohammad Amir |
| title |
Determination of bacterial and viral pathogen spectra and identification of multi drugs resistance gene CTX-M1 in Klebsiella pneumoniae isolated from acute respiratory infections children aged under-5 from two hospital settings of Dhaka city |
| title_short |
Determination of bacterial and viral pathogen spectra and identification of multi drugs resistance gene CTX-M1 in Klebsiella pneumoniae isolated from acute respiratory infections children aged under-5 from two hospital settings of Dhaka city |
| title_full |
Determination of bacterial and viral pathogen spectra and identification of multi drugs resistance gene CTX-M1 in Klebsiella pneumoniae isolated from acute respiratory infections children aged under-5 from two hospital settings of Dhaka city |
| title_fullStr |
Determination of bacterial and viral pathogen spectra and identification of multi drugs resistance gene CTX-M1 in Klebsiella pneumoniae isolated from acute respiratory infections children aged under-5 from two hospital settings of Dhaka city |
| title_full_unstemmed |
Determination of bacterial and viral pathogen spectra and identification of multi drugs resistance gene CTX-M1 in Klebsiella pneumoniae isolated from acute respiratory infections children aged under-5 from two hospital settings of Dhaka city |
| title_sort |
determination of bacterial and viral pathogen spectra and identification of multi drugs resistance gene ctx-m1 in klebsiella pneumoniae isolated from acute respiratory infections children aged under-5 from two hospital settings of dhaka city |
| publisher |
Brac University |
| publishDate |
2019 |
| url |
http://hdl.handle.net/10361/12083 |
| work_keys_str_mv |
AT hossainmohammadamir determinationofbacterialandviralpathogenspectraandidentificationofmultidrugsresistancegenectxm1inklebsiellapneumoniaeisolatedfromacuterespiratoryinfectionschildrenagedunder5fromtwohospitalsettingsofdhakacity |
| _version_ |
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