In silico design of a capsid vertex component 1 protein targeted multi-epitope vaccine against cytomegalovirus
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2024.
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| 言語: | English |
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Brac University
2024
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| オンライン･アクセス: | http://hdl.handle.net/10361/23697 |
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10361-236972024-07-15T21:02:17Z In silico design of a capsid vertex component 1 protein targeted multi-epitope vaccine against cytomegalovirus Nabin, Md. Naimur Rahman Siam, Mohammad Kawsar Sharif School of Pharmacy, Brac University Cytomegalovirus Multi-epitope vaccine Capsid vertex component 1 Biochemical analysis Cytomegalovirus infections--Treatment This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2024. Cataloged from the PDF version of thesis. Includes bibliographical references (pages 55-60). Cytomegalovirus is a virus causing irreversible neurological damage in newborns. In this study, in silico computational method was performed where, as primary protein, Capsid Vertex Component 1 Protein of cytomegalovirus from Uniprot server was selected. Using Vaxijen v2.0 it’s antigenicity (0.5142) & Cytotoxic T lymphocyte,helper T lymphocyte,B cell epitopes was found via NetCTL-1.2, NetMHCIIpan 4.0, Bepipred servers. Helper T lymphocyte epitopes were Screened via IFN, IL-4Pred & IL-10Pred epitopes. For constructing the vaccine, linkers were used. A Biochemical analysis gave potential results. With ProtParam the instability index (34.10), grand average of hydropathy (-0.263) & molecular weight (56399.91 kDa) was predicted. Using AllergenOnline & T3DB, the vaccine was discerned as non-allergen & non-toxic. ProSAweb gave Z-score (-5.95) & SWISS-MODEL generated Ramachandran plots. By C-IMMSIM, desirable responses of immune cells & antibodies in accordance with three doses of the vaccine was analyzed. In vitro, in vivo methods can be done to validate the safety & efficacy of the vaccine. Md. Naimur Rahman Nabin B. Pharmacy 2024-07-15T05:58:25Z 2024-07-15T05:58:25Z ©2024 2024-02 Thesis ID 19346041 http://hdl.handle.net/10361/23697 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 74 pages application/pdf Brac University |
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Brac University |
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Institutional Repository |
| language |
English |
| topic |
Cytomegalovirus Multi-epitope vaccine Capsid vertex component 1 Biochemical analysis Cytomegalovirus infections--Treatment |
| spellingShingle |
Cytomegalovirus Multi-epitope vaccine Capsid vertex component 1 Biochemical analysis Cytomegalovirus infections--Treatment Nabin, Md. Naimur Rahman In silico design of a capsid vertex component 1 protein targeted multi-epitope vaccine against cytomegalovirus |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2024. |
| author2 |
Siam, Mohammad Kawsar Sharif |
| author_facet |
Siam, Mohammad Kawsar Sharif Nabin, Md. Naimur Rahman |
| format |
Thesis |
| author |
Nabin, Md. Naimur Rahman |
| author_sort |
Nabin, Md. Naimur Rahman |
| title |
In silico design of a capsid vertex component 1 protein targeted multi-epitope vaccine against cytomegalovirus |
| title_short |
In silico design of a capsid vertex component 1 protein targeted multi-epitope vaccine against cytomegalovirus |
| title_full |
In silico design of a capsid vertex component 1 protein targeted multi-epitope vaccine against cytomegalovirus |
| title_fullStr |
In silico design of a capsid vertex component 1 protein targeted multi-epitope vaccine against cytomegalovirus |
| title_full_unstemmed |
In silico design of a capsid vertex component 1 protein targeted multi-epitope vaccine against cytomegalovirus |
| title_sort |
in silico design of a capsid vertex component 1 protein targeted multi-epitope vaccine against cytomegalovirus |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23697 |
| work_keys_str_mv |
AT nabinmdnaimurrahman insilicodesignofacapsidvertexcomponent1proteintargetedmultiepitopevaccineagainstcytomegalovirus |
| _version_ |
1814308273219698688 |