Determination of antibacterial and antioxidant activity of Spondias mombin stem bark extracts
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024.
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Brac University
2024
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10361-237702024-08-14T21:06:23Z Determination of antibacterial and antioxidant activity of Spondias mombin stem bark extracts Saiyara, Fabiha Hussain, Humyra Binte Hossain, M. Mahboob Department of Mathematics and Natural Sciences, Brac University Spondias mombin Antibacterial Antioxidant Thin layer chromatography Disk diffusion DPPH assay Biotechnology This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024. Catalogued from PDF version of thesis. Includes bibliographical references (pages 51-55). Plants have been used throughout history in traditional medicine, and remain a promising source of pharmaceuticals. Spondias mombin, an ethnomedicinal plant recognized for its efficacy in treating various diseases, is also the source of numerous therapeutic agents. Previous studies on the bark of this plant revealed its abundance of beneficial compounds along with several pharmacological properties. Although widely available in Bangladesh, limited scientific studies have been reported on this plant. Therefore, this thesis aims to investigate the antibacterial and antioxidant activity of locally sourced S. mombin stem bark extracts. The ethanolic bark extract was subjected to solvent partitioning with chloroform, ethyl acetate, hexane, and methanol. Then thin-layer chromatography, Kirby-Bauer disk diffusion test, and DPPH free radical scavenging assay were conducted on the solvent partitions. The ethyl acetate partition extract exhibited mild antibacterial activity against Acinetobacter baumanni ATCC 19606, E. coli ATCC 25922, Klebsiella pneumonia and Salmonella typhi. Additionally, all four partition extracts displayed very strong antioxidant activities, with the ethyl acetate extract demonstrating the strongest activity and the lowest IC50 value of 4.74 μg/mL. Visualization of the TLC plates showed the presence of several compounds in the chloroform, hexane, and ethyl acetate partitions which may be of medicinal value. Therefore, these findings underscore the importance of further research into this plant for therapeutic purposes. Fabiha Saiyara Humyra Binte Hussain B. Biotechnology 2024-08-14T06:28:08Z 2024-08-14T06:28:08Z 2024 2024-04 Thesis ID 19236005 ID 19236019 http://hdl.handle.net/10361/23770 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 55 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Spondias mombin Antibacterial Antioxidant Thin layer chromatography Disk diffusion DPPH assay Biotechnology |
| spellingShingle |
Spondias mombin Antibacterial Antioxidant Thin layer chromatography Disk diffusion DPPH assay Biotechnology Saiyara, Fabiha Hussain, Humyra Binte Determination of antibacterial and antioxidant activity of Spondias mombin stem bark extracts |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024. |
| author2 |
Hossain, M. Mahboob |
| author_facet |
Hossain, M. Mahboob Saiyara, Fabiha Hussain, Humyra Binte |
| format |
Thesis |
| author |
Saiyara, Fabiha Hussain, Humyra Binte |
| author_sort |
Saiyara, Fabiha |
| title |
Determination of antibacterial and antioxidant activity of Spondias mombin stem bark extracts |
| title_short |
Determination of antibacterial and antioxidant activity of Spondias mombin stem bark extracts |
| title_full |
Determination of antibacterial and antioxidant activity of Spondias mombin stem bark extracts |
| title_fullStr |
Determination of antibacterial and antioxidant activity of Spondias mombin stem bark extracts |
| title_full_unstemmed |
Determination of antibacterial and antioxidant activity of Spondias mombin stem bark extracts |
| title_sort |
determination of antibacterial and antioxidant activity of spondias mombin stem bark extracts |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23770 |
| work_keys_str_mv |
AT saiyarafabiha determinationofantibacterialandantioxidantactivityofspondiasmombinstembarkextracts AT hussainhumyrabinte determinationofantibacterialandantioxidantactivityofspondiasmombinstembarkextracts |
| _version_ |
1814309734139822080 |