Diversity of salmonella typhi-phages in Dhaka city through whole genome sequencing
This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Master of Science in Biotechnology, 2024.
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| التنسيق: | أطروحة |
| اللغة: | English |
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Brac University
2024
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10361/24007 |
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10361-24007 |
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10361-240072024-09-10T10:47:49Z Diversity of salmonella typhi-phages in Dhaka city through whole genome sequencing Islam, Shuborno Naser, Iftekhar Bin Department of Mathematics and Natural Sciences, Brac University Bacteriophages Salmonella Typhi Genome Sequencing Phage diversity Bacteriophage ecology. Bacteriophages--Genetics--Bangladesh--Dhaka. This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Master of Science in Biotechnology, 2024. Cataloged from PDF version of thesis. Includes bibliographical references (pages 54-60). Bacteriophages and their hosts form a vast ecosystem, with bacteria evolving diverse defense mechanisms against phage infection while phages simultaneously develop strategies to overcome these defenses. Limited data on Salmonella Typhi phages hampers understanding of their genomics, evolution, and interactions with bacterial hosts. This dissertation aims to study Salmonella Typhi phages in the environmental water sources of Dhaka city, focusing on their genomic characterization and structure. Phage DNA was sequenced using a combination of propagation experiments, genomic DNA extraction, and sequencing library preparation. Computational tools, including Pharokka and Kraken2, were employed to analyze the genetic makeup and taxonomical classification of these phages. A phylogenetic tree was generated using tail fiber protein sequences to explore evolutionary relationships with mid-20th-century phages. Recent findings indicate the circulation of three phage genera, with Kayfunavirus being dominant, followed by Teseptimavirus and Macdonaldcampvirus. The generated genome map revealed gene structures and arrangements, highlighting annotated proteins required by the phages and further denoted large sequences of hypothetical proteins that can be crucial for phage survival and host interaction. These insights contribute to understanding pathogen behavior, the spread of antibiotic-resistant genes, and environmental survival mechanisms, underscoring the importance of phages in microbial ecology. Shuborno Islam M. Biotechnology 2024-09-08T06:52:26Z 2024-09-08T06:52:26Z ©2024 2024-06 Thesis ID 22376001 http://hdl.handle.net/10361/24007 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 60 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Bacteriophages Salmonella Typhi Genome Sequencing Phage diversity Bacteriophage ecology. Bacteriophages--Genetics--Bangladesh--Dhaka. |
| spellingShingle |
Bacteriophages Salmonella Typhi Genome Sequencing Phage diversity Bacteriophage ecology. Bacteriophages--Genetics--Bangladesh--Dhaka. Islam, Shuborno Diversity of salmonella typhi-phages in Dhaka city through whole genome sequencing |
| description |
This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Master of Science in Biotechnology, 2024. |
| author2 |
Naser, Iftekhar Bin |
| author_facet |
Naser, Iftekhar Bin Islam, Shuborno |
| format |
Thesis |
| author |
Islam, Shuborno |
| author_sort |
Islam, Shuborno |
| title |
Diversity of salmonella typhi-phages in Dhaka city through whole genome sequencing |
| title_short |
Diversity of salmonella typhi-phages in Dhaka city through whole genome sequencing |
| title_full |
Diversity of salmonella typhi-phages in Dhaka city through whole genome sequencing |
| title_fullStr |
Diversity of salmonella typhi-phages in Dhaka city through whole genome sequencing |
| title_full_unstemmed |
Diversity of salmonella typhi-phages in Dhaka city through whole genome sequencing |
| title_sort |
diversity of salmonella typhi-phages in dhaka city through whole genome sequencing |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/24007 |
| work_keys_str_mv |
AT islamshuborno diversityofsalmonellatyphiphagesindhakacitythroughwholegenomesequencing |
| _version_ |
1814307957718908928 |