Isolation and characterization of potential bacteria responsible for biodegradation of low-density polyethylene collected from garbage dump sites in Dhaka
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2022.
| Main Authors: | , |
|---|---|
| 其他作者: | |
| 格式: | Thesis |
| 語言: | English |
| 出版: |
Brac University
2024
|
| 主題: | |
| 在線閱讀: | http://hdl.handle.net/10361/24370 |
| id |
10361-24370 |
|---|---|
| record_format |
dspace |
| spelling |
10361-243702024-10-22T21:01:54Z Isolation and characterization of potential bacteria responsible for biodegradation of low-density polyethylene collected from garbage dump sites in Dhaka Nahar, Abida Nurun Farzana, Maisha Akhter, Fahmina Haque, Munima Department of Mathematics and Natural Sciences, Brac University Biodegradation Bacillus Environmental biotechnology Isolation Polyethylene--Environmental aspects. Bacteria--Isolation and purification. Microbial biodegradation. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 63-70). Being a multifaceted polymer, the recalcitrant Low Density Polyethylene (LDPE) has slowly gained popularity for ease of access while simultaneously becoming a threat to nature. This is why investigating diverse bacteria and exploring their natural capability to biodegrade LDPE has become a pressing matter in environmental biotechnology research. This study explores the LDPE biodegradation potential of bacteria isolated from half-degraded plastics from garbage dump sites. Significant weight loss (20%-30%) was detected from five isolated bacteria from pure cultures. 16sRNA gene sequence of three of them revealed them to be Bacillus sp. (in: Bacteria) strain JDMASP60, Bacillus atrophaeus strain MGB14, and Bacillus pumilus strain KD3 indicating their taxonomic novelty. Additional biodegradation examinations revealed their LDPE metabolization capability. Optical microscope analysis at 1000x magnification showed substantial amount of damage (cracks, grooves, and minor holes) on the polymer surface. FTIR showed a percentage increase in transmittance at the peaks 2918 cm^-1, 2852 cm^-1, 1468 cm^-1, 1373 cm^-1, 718 cm^-1, among many others indicating a decrease in native bonds. Moreover, a new peak corresponding to aromatic C=C bond at 1600cm^cm-1 was also reported. The study highlighted the biodegrading capability of naturally occurring bacteria at garbage dump sites while using LDPE as their sole carbon source. Abida Nurun Nahar Maisha Farzana B.Sc. in Biotechnology 2024-10-22T05:41:53Z 2024-10-22T05:41:53Z ©2022 2022-08 Thesis ID 18136030 ID 18136089 http://hdl.handle.net/10361/24370 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 70 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Biodegradation Bacillus Environmental biotechnology Isolation Polyethylene--Environmental aspects. Bacteria--Isolation and purification. Microbial biodegradation. |
| spellingShingle |
Biodegradation Bacillus Environmental biotechnology Isolation Polyethylene--Environmental aspects. Bacteria--Isolation and purification. Microbial biodegradation. Nahar, Abida Nurun Farzana, Maisha Isolation and characterization of potential bacteria responsible for biodegradation of low-density polyethylene collected from garbage dump sites in Dhaka |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2022. |
| author2 |
Akhter, Fahmina |
| author_facet |
Akhter, Fahmina Nahar, Abida Nurun Farzana, Maisha |
| format |
Thesis |
| author |
Nahar, Abida Nurun Farzana, Maisha |
| author_sort |
Nahar, Abida Nurun |
| title |
Isolation and characterization of potential bacteria responsible for biodegradation of low-density polyethylene collected from garbage dump sites in Dhaka |
| title_short |
Isolation and characterization of potential bacteria responsible for biodegradation of low-density polyethylene collected from garbage dump sites in Dhaka |
| title_full |
Isolation and characterization of potential bacteria responsible for biodegradation of low-density polyethylene collected from garbage dump sites in Dhaka |
| title_fullStr |
Isolation and characterization of potential bacteria responsible for biodegradation of low-density polyethylene collected from garbage dump sites in Dhaka |
| title_full_unstemmed |
Isolation and characterization of potential bacteria responsible for biodegradation of low-density polyethylene collected from garbage dump sites in Dhaka |
| title_sort |
isolation and characterization of potential bacteria responsible for biodegradation of low-density polyethylene collected from garbage dump sites in dhaka |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/24370 |
| work_keys_str_mv |
AT naharabidanurun isolationandcharacterizationofpotentialbacteriaresponsibleforbiodegradationoflowdensitypolyethylenecollectedfromgarbagedumpsitesindhaka AT farzanamaisha isolationandcharacterizationofpotentialbacteriaresponsibleforbiodegradationoflowdensitypolyethylenecollectedfromgarbagedumpsitesindhaka |
| _version_ |
1814307831340335104 |