05568pam a2200685 i 4500 AALScienceDirectebook000009 BD-DhAAL 20191205134905.0 m o d cr cnu|||unuuu 150928s2016 ne ob 001 0 eng d 41954 41954 0081001142 9780081001141 0081000944 9780081000946 (OCoLC)922323918 (OCoLC)922918604 (OCoLC)929521751 (OCoLC)932328797 (OCoLC)951666431 (OCoLC)1066557439 BD-DhAAL RM301.25 2015 L-371 QV 38 MED 071000 bisacsh 615.1901 23 Concepts and models for drug permeability studies : cell and tissue based In vitro culture models / edited by Bruno Sarmento. [electronic resource] Amsterdam : Elsevier, 2016. �2016 1 online resource. Woodhead Publishing series in biomedicine ; number 79 <strong>Off-Campus Access:</strong> Athens ID and Password Required <strong>On-Campus Access:</strong> No User ID or Password Required Includes bibliographical references and index. Front Cover; Related titles; Concepts and Models for Drug Permeability Studies; Copyright; Contents; List of contributors; List of figures; List of tables; 1 -- Introduction; 1.1 Introduction; References; 2 -- Importance and applications of cell- and tissue-based in vitro models for drug permeability screening in early stages o ...; 2.1 Introduction; 2.2 General considerations; 2.3 Drug transport; 2.3.1 Transport mechanisms; 2.4 Permeability-absorption models; 2.4.1 Physicochemical methods; 2.4.1.1 Physicochemical factors; 2.4.1.2 Immobilized artificial membrane chromatography 2.4.1.3 Parallel artificial membrane permeability assay2.4.2 In vitro cell and tissue methods; 2.4.2.1 Cell-based methods; 2.4.2.2 Tissue-based methods; Diffusion chambers; Franz cells; Everted sacs; Isolated membrane vesicles; 2.5 Methods for permeability calculation; 2.6 Standardization of protocols for in vitro methods; 2.7 The "three Rs" principle; 2.8 Biosecurity systems; References; 3.1 -- Cell-based in vitro models for buccal permeability studies; 3.1.1 Introduction; 3.1.2 Physiology of the buccal mucosa; 3.1.3 Different in vitro models; 3.1.3.1 Hamster cheek pouch cells 3.1.3.2 TR146 cell line3.1.3.2.1 Protocol; Cell culture conditions; Permeability studies; 3.1.3.3 Human oral keratinocytes; 3.1.3.3.1 Protocol; Oral keratinocyte culture conditions; Culturing on dead de-epidermized dermis (DDED); Permeability studies; 3.1.3.4 MatTek EpiOral"; 3.1.3.4.1 Protocol; Cell culture conditions; Permeability studies; 3.1.4 Conclusions; References; 3.2 -- Cell-based in vitro models for gastric permeability studies; 3.2.1 The stomach as a natural barrier to absorption; 3.2.2 Gastric drug delivery; 3.2.2.1 Molecular absorption in the stomach 3.2.2.2 Physicochemical factors mediating stomach absorptive permeability3.2.3 Cellularized models of gastric permeability; 3.2.3.1 Protocol for establishing cellularized artificial models of the gastric wall; 3.2.4 Conclusions; Acknowledgments; References; 3.3 -- Cell-based in vitro models for intestinal permeability studies; 3.3.1 Anatomy and physiology of human small intestine; 3.3.1.1 Stromal-epithelial cross-talk; 3.3.2 Mechanisms of transport; 3.3.3 Intestinal barriers; 3.3.4 Intestinal in vitro models; 3.3.4.1 Caco-2 model; 3.3.4.1.1 Accelerated Caco-2 models 3.3.4.1.2 Alternatives to the Caco-2 model3.3.4.2 Caco-2/HT29-MTX model; 3.3.4.3 Caco-2/Raji B model; 3.3.4.4 Caco-2/HT29-MTX/Raji B; 3.3.4.5 Novel 3D in vitro models; 3.3.5 Validation studies; 3.3.6 Conclusions; References; 3.4 -- Cell-based in vitro models for nasal permeability studies; 3.4.1 Introduction; 3.4.2 Nasal primary cell culture models; 3.4.2.1 Sampling approaches and procedures; 3.4.2.2 HNE cell preparation and culture initiation; 3.4.3 Immortalized nasal cell lines; 3.4.3.1 RMPI 2650 cell line; 3.4.3.2 BT cell line; 3.4.3.3 Human lung carcinoma cell line Electronic access restricted to authorized BRAC University faculty, staff and students Mode of access: Internet Cell culture. 38039 Drugs Physiological transport Research Methodology. 38040 Drugs Testing. 38041 Epithelial cells. 38042 Cell culture. fast 38039 Drugs Testing. fast 38041 Epithelial cells. fast 38042 MEDICAL / Pharmacology bisacsh 38043 Permeability. 38044 Pharmacokinetics. 38045 Cell Culture Techniques. 38046 Models, Biological. 38047 Pharmaceutical Preparations metabolism. 38048 Tissue Culture Techniques. 38049 Electronic books. 38050 Electronic books. 38050 Sarmento, Bruno, editor. 38051 Print version: 9780081000946 0081000944 (OCoLC)911073450 Woodhead Publishing series in biomedicine ; no. 79. 38052 Full text available on ScienceDirect http://www.sciencedirect.com/science/book/9780081000946 ddc EBK 0 0 ddc 0 615_190100000000000_CON 0 67377 BRACUL BRACUL 2019-12-05 0 615.1901 CON 2019-12-05 2019-12-05 EBK