06398nam a22005178i 4500 44451 BD-DhAAL 20250721155119.0 250616t2021 njuab b 001 0 eng 2020029236 9781119640783 9781119640752 9781119640677 DNLM/DLC eng rda DLC BD-DhAAL pcc QH442.2 572.8633 23 Brown, T. A. (Terence A.), author. Gene cloning and DNA analysis : an introduction / Terry A. Brown. Eighth edition. Hoboken, NJ : Wiley-Blackwell, 2021. xv, 416 pages : illustrations (chiefly color), color maps ; 25 cm. Includes bibliographical references and index. Why gene cloning and DNA analysis are important -- Vectors for gene cloning : plasmids and bacteriophages -- Purification of DNA from living cells -- Manipulation of purified DNA -- Introduction of DNA into living cells -- Cloning vectors for Escherichia coli -- Cloning vectors for eukaryotes -- How to obtain a clone of a specific gene -- The polymerase chain reaction -- Sequencing genes and genomes -- Studying gene expression and function -- Studying genomes -- Studying transcriptomes and proteomes -- Production of protein from cloned genes -- Gene cloning and DNA analysis in medicine -- Gene cloning and DNA analysis in agriculture -- Gene cloning and DNA analysis in forensic science and archaeology. "The main development in DNA technology since publication of the Seventh Edition of Gene Cloning has been the increased use of gene editing as a tool in both research and biotechnology. The basic methodology for CRISPR editing is now described in Chapter 12 and the applications of the method are explored, in the context of plant genetic engineering, in Chapter 16. Elsewhere, the continuing evolution of next-generation DNA sequencing is reflected by a reorganization of this part of Chapter 10, and to deal with the further proliferation of methods for studying transcriptomes and proteomes (albeit not strictly DNA Analysis) I have created a new chapter devoted to these methods. Other additions include new sections on melt curve analysis of real-time PCR products and genetic typing of human disease mutations"-- MNS Dr. Nadia Sultana Deen Associate Professor Department of Mathematics and Natural Sciences Course name and No.: Microbial Genetic Engineering (MIC 401) Karim International 44451, 44452, 44453, 44454, 44455, 44456, 44457, 44458, 44459, 44501 Cloning, Molecular. Sequence Analysis, DNA. DNA, Recombinant Analysis. DNA Analysis. Nucleotide sequence. Molecular cloning. 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